CD Tesis
Biodegradasi Metilen Biru Oleh Fungi Hasil Isolasi Dari Tanah Terkontaminasi Limbah Minyak Bumi
Due to rapid development of dye and dye-related industries, a large number of dyes are manufactured and are being used almost every day. About 280,000 tons of dyes are discharged as waste every year. The waste of dyes have an adverse effect on water environment like depletion of dissolved oxygen which increase biological oxygen demand and death of aquatic life. One of the acceptable methods with low cost to degrade dye pollutants is degradation by fungi. Generally, fungi have an extracellular enzyme (i.e., laccase, lignin peroxidase and manganese peroxidase) that are able to degrade dyes.
In this research, eight isolates of fungi isolated from oil contaminated soil were used to degrade methylene blue. The initial screening showed that three fungi strains have high activity to degrade methylene blue. These fungi were called as FTM2 (Penicillium sp.), FTM6 (Aspergillus sp.) and FTM7 (Penicillium sp.). Furthermore, the activity to degrade methylene blue of these fungi strains was determined by the spectrophotometry method. The effect of various physicochemical conditions like contact time, initial concentration, agitation and pH on degradation of methylene blue was studied. The results showed that FTM7 (Penicillium sp.) has greatest activity to degrade methylene blue. The optimum degradation of MB by Penicillium sp. is found after 8 days incubation with agitation 150 rpm and the concentration of MB is 40 ppm and unadjusted pH (95.45%). Also, the degradation of MB was found to follow first-order reaction kinetic.
Keywords: Biodegradation, Methylene Blue, Fungi.
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