CD Skripsi

Pengaruh Penambahan Ion Logam Mg2+, Ca2+ Dan Mn2+ Terhadap Pertumbuhan Jamur Trichoderma Asperellum Lbkurcc1

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Laccase (EC 1.10.3.2, p-diphenol: oxygen oxidoreductase) is a multicopper enzyme
(containing 4 copper ions) which is one of the extracellular enzymes that are widely
used in the process of lignin degradation. Laccase is classified as an oxidoreductase
enzyme capable of oxidizing both aromatic and non aromatic compounds. Laccase
enzyme can be found by fungi. One of the fungi that can produce laccase enzymes is
T. asperellum. This study aims to determine the effect of the addition of metal ions to
laccase enzyme activity using of 2,2-azinobis (3- ethylbenzatiazoline) -6-sulfonate
(ABTS) as a substrate. The metal ions being added for laccase production are Mg2+,
Mn2+ and Ca2+ with each concentrations is 2 mM. The laccase enzyme activity assay
was measured using a UV-Vis spectrophotometer with a wavelength of 420 nm and
incubated for 0, 5 and 10 minutes. The highest laccase enzyme activity was obtained
at 5 minutes incubation for the addition of Ca2+ metal ions, with the value is 3,7064 x
10-3 ± 2.1215 x 10-3 U/mL which is able to increase lacasse activity by 73,97%.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 121 (0039)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2021

Deskripsi Fisik

xiii, 37 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 121 (0039)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

-

Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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