CD Skripsi
Identifikasi Metabolisme Sel Alga Biosensor Berdasarkan Pengukuran Impedansi
The decline in the quality of aquatic resources due to industrial and agricultural
activities and is a threat and impact to existing organisms in the water. Algae are
the largest oxygen producers and play an important role in the quality of the
waters, the vitality of algae can be seen by the increase in oxygen that the algae
emits during photosynthesis. Based on this, in this study the algae Chlorella sp. as
a bioreceptor will be evaluated the value of impedance as a function of the density
() of the population and the influence of artificial light LED as a light source to
the level of vitality of algae in producing oxygen resulting from photosynthesis,
where the higher the oxygen produced the better the quality of a water. Electronic
circuit impedance with excitation source of PCI 6251 National Instrument multi
I/O Card based on LabVIEW software that will measure impedance, real,
imaginary value and phase angle of algae. The process of measuring algae
impedance is done by injecting alternating current (AC) using platinum electrodes
where the frequency parameters start from 10 Hz – 50 kHz. Measurements are
performed by varying the density of algae (), the wavelength of the LED (λ) and
the intensity of the red LED (I0). The results explain that red light (650 nm – 750
nm) is more absorbed by algae than illuminated by blue light (400 nm – 450 nm)
and impedance will increase with increasing () and (I0). The level of vitality of
algae when illuminated by 3 red LEDs gets a higher impedance value compared to
illuminated with 2 red LEDs and 1 red LED. Real value measurements get
improved results while imaginary value measurements get relatively decreased
results and negative values for capacitive samples. Phase angle measurement
using variations (), (λ) and (I0) at a frequency of 10 Hz gets a maximum value of
-68.98 to -69.35 angles with the angle value being in quadrant IV. Measurements
are performed by varying the amplitude of THE PC's voltage against impedance
with variations of 100 mV, 300 mV, 500 mV and 1 V getting the result of
impedance values increasing as the voltage amplitude value increases.
Keywords: Algae, Impedance, Artificial Light
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