CD Skripsi

Deteksi Pemanis Buatan Menggunakan Metabolisme Ragi Saccharomyces Cerevisiae Sebagai Reseptor Biosensor

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The consumption of sugar in food and beverages in recent years has increased
significantly, so that it can increase the risk of various diseases. Based on this, a
method to monitor and minimize the risk of the disease is needed. In this study,
Saccharomyces cerevisiae yeast was used as a bioreceptor using a Clark type
oxygen sensor based on Pt-Ag electrodes with the principle of amperometric
biosensor. The results of yeast cell metabolic activity were observed by adding
sugar samples in the form of cellular respiration which was expressed as the level
of dissolved oxygen (DO). The samples used in this study were natural sugar
sucrose, fructose and artificial sweetener aspartame with various concentrations of
0.1 M, 0.3 M and 0.5 M. The DO levels produced by the addition of natural sugar
sucrose for concentrations of 0.1 M, 0.3 M and 0.5 M were 27,55%, 11,52% and
2,36%, while the fructose concentrations of 0.1 M, 0.3 M and 0.5 M were 30.77%,
17.29% and 5.28%. The addition of artificial sweeteners resulted in DO levels for
concentrations of 0.1 M, 0.3 M and 0.5 M were 26,43%, 20,06% and 11,76%. The
measurement results show that the sensor electrodes can detect changes that occur
due to the addition of natural sugars and artificial sweeteners from each
concentration that affect the respiratory activity of the interaction of yeast
bioreceptors and sugar analytes.
Keywords: Saccharomyces cerevisiae, amperometric biosensor, dissolved oxygen,
sucrose, fructose, aspartame

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 01.121 (0062)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – FISIKA., 2021

Deskripsi Fisik

xv, 60 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 01.121 (0062)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

FISIKA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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