CD Tesis
Respons Fisiologis Dan Histopatologi Ikan Patin Siam (Pangasianadon Hypophthalmus) Yang Dipelihara Pada Salinitas Berbeda
This study was conducted from Desember 2020 to February 2021 and that
locate was Fish Parasites and Diseases, and Marine Science Technology Biology,
Faculty of Fisheries and Marine, Riau University. This study aims to analyze the
physiological and histopathological responses of Pangasianodon hypophthalmus
reared at different salinities. The method used is an experimental method by
applying a one-factor Completely Randomized Design (CRD) with four treatment
levels and three replications. The treatments in this study were 1) (0 ppt), 2) (5
ppt), 3 (7 ppt), and 4) (9 ppt). The fish used measuring 8-10 cm were kept in an
aquarium measuring 60x30x30cm with a stocking density of 1 fish/2 L (20
fish/aquarium) for 45 days. Feeding was carried out 3 times a day, namely at
08.00, 12.00 and 18.00 WIB at satation. Observations on the level of osmotic
work, oxygen consumption level, total erythrocytes, total leukocytes, and blood
glucose were carried out at the beginning of maintenance, the 23rd day of
maintenance and 45th day of maintenance, weight and length measurements were
carried out once every 10 days, while the histopathological sampling of organs
was carried out. gills and kidneys were carried out on the 45th day of
maintenance. While histopathological sampling of gill and kidney organs was
carried out on the 45th day of maintenance.
The results showed that the maintenance of P. hypophthalmus in different
salinity media had a significant effect on the physiology of P. hypophthalmus. 5
ppt salinity is an effective salinity for the maintenance of P. hypophthalmus, seen
from the osmotic work rate of 21,66 mOsm/I H2O, oxygen consumption rate of
0,410 mgO2/g body weight/hour, absolute weight growth 9,63 g, absolute length
3,54 cm, LPS 2,99%, total erythrocytes 2,25 x106 cells/mm3, total leukocytes 7,65
x104 cells/mm3, blood glucose 52,33 mg/dL, survival rate 100%, and water quality
values ranging from 28,1-28,5oC, pH ranged from 6-6,5, DO ranged from 5,9-6,5
mg/L, ammonia ranged from 0,004-0,051 mg/L. Histology of gills and kidneys
with a salinity of 5 ppt showed normal tissue conditions.
Key words: Pangasianodon hypophthalmus, Salinity, Physiological, Histological
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