CD Skripsi
Optimasi Media Pertumbuhan Bakteri Bacillus Cereus Dengan Penambahan Susu Skim Dan Sumber Karbon Berbeda
ABSTRACT
The goal of this research was to find the optimal media for growing B.
cereus bacteria with various carbon sources. This study used an experimental
method with two Bacillus cereus bacterium isolates, namely B. cereus strain SN7
(isolate N) and a consortium of bacteria isolates (a combination of several B.
cereus isolates with different strains). The two isolates were grown in sago liquid
waste media, tofu liquid waste media, and molasses liquid waste media
supplemented with a protein source, skim milk, with three replications in each
treatment. Bacterial culture growth was measured every 6 hours for 24 hours
using two methods: the TPC method and bacterial cell biomass measurement.
According to the results of this study's measurements, molasses waste media was
the best modified medium for B. cereus growth. The difference in growth between
the B. cereus strain SN7 bacterial isolates and the consortium cultured on
molasses media was not significant. The isolates with the largest number of cell
growth were B. cereus consortium isolates, with 2,023 x108 CFU/ml in the TPC
method and 1,011 g/ml in the biomass measurement method at 24 hours.
Keywords: Bacillus cereus, carbon, molasses, optimal growth, skim milk
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