CD Disertasi

Optimasi Pemanfaatan Lindi Sampah Perkotaan Untuk Kultur Chlorella Sp

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The increasing amount of waste produced in big cities is a
very serious problem that must be solved on an ongoing basis. Waste undergoes a
decomposition process which eventually changes physically, chemically and biologically.
The result of the decomposition process is leachate. Leachate contains a lot of nutrients
needed by plants, including microalgae. To optimize the use of leachate, it can be used
for microalgae culture of Chlorella sp. This study aimed to analyze: Optimal use of
leachate either with or without filtration and different concentration levels towards the
growth and abundance of Chlorella sp cells; proximate and amino acids content of
Chlorella sp cells; macronutrients contents and micronutrients. Materials and Methods:
This study used an experimental method, completely randomized design with 1 factor and
5 levels of treatment and three replications. The research consisted of two stages of
research, namely: 1). Study of leachate use without filtration with different concentrations
towards the growth and abundance of Chlorella sp. The levels of leachate concentration
were; (P1 = 5% leachate concentration, P2 = 10%, P3 = 15%, and P4 = 20% and P5 =
25%/L water); 2). Study of leachate use by filtration and different concentrations towards
the growth and abundance of Chlorella sp. The levels of leachate concentration were; (P1
= 5% leachate concentration, P2 = 10%), P3 = 15%, and P4 = 20% and P5 = 25%/L
water). The research data were analyzed according to ANOVA statistics (P < 0.05), then
further tested with Newman Keuls using SPSS 17.0. Results and Discussion: Utilization
of leachate without filtration with a concentration of 10% was the optimal value, resulting
in the highest growth and abundance of Chlorella sp cells, namely 5516.7x103 cells/mL,
peak population on day 12. The use of leachate with 10% concentration filtration resulted
in the highest cell abundance of 2250x103 cells/mL, the peak of the population on the
10th day. Utilization of leachate without filtration obtained protein content of 30.27-
32.82%, fat 7.84-8.46% and carbohydrates 27.65-30.02%. For leachate by filtration, the
protein content was of 29.67-31.01%, fat was of 6.92-8.61% and carbohydrates was of
28.29-33.17%. On unfiltered leachate, there were 8 non-essential amino acids, whereas in
filtered leachate there were 8 essential amino acids and 9 non-essential amino acids. The
macronutrients in the leachate without filtration were nitrate and phosphate. The nitrate
contents at the beginning and end of the measurement were of 1.186-2.075 mg/L and
0.804-1.085 mg/L, whereas the initial and final values of phosphate were 0.047-0.153
mg/L. and 4.075-4.148 mg/L. The final contents of heavy metal elements such as Cu, Fe,
Zn and Mn were below the water quality standard according to the Regulation of
Environment and Forestry Minister No.59/2016, and the content of heavy metal element
Cd was below the quality standard of the Ministry of Agriculture No.70/2011.
Conclusion: Utilization of leachate without filtration with a concentration of 10% was
the optimal value, resulting in the highest growth and abundance of Chlorella sp cells of
5516.67x103 cells/mL, with the highest specific growth rate (k) of 0.198/day, and total
biomass of 0, 24 (g/L). There was no significant difference in the value of proximate
content of leachate either with or without filtration. Therefore, the contents of
macronutrients (nitrate, phosphate) and micronutrients (Cu, Fe, Zn and Mn and Cd) of
leachate support the growth of microalgae Chlorella sp culture.
Keywords: Chlorella sp, leachate, abundance, filtration.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 03. 322 (0001)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Disertasi Ilmu Kelautan., 2022

Deskripsi Fisik

xxv, 240 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 03. 322 (0001)

Tipe Isi

-

Tipe Media

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Tipe Pembawa

-

Edisi

-

Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

Lampiran Berkas

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