CD Skripsi

Isolasi, Seleksi Dan Uji Potensi Lipase Bakteri Lipolitik Asal Limbah Cair Industri Kelapa Sawit Sebagai Penyusun Biodetergen

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ABSTRACT
Lipolytic bacteria are bacteria that produce lipase enzymes to hydrolyze fats into fatty acids and glycerol. Lipolytic bacteria can be found in oil-contaminated habitats such as palm oil mill effluent. One of the applications of lipase enzymes is as biodetergents formulation that are environmentally friendly. This research aims to obtain potential lipolytic bacteria isolates from palm oil mill effluent and see the potential of their lipase for application as a biodetergent formulation through several tests, enzyme activity assay, enzyme activity stability test based on temperature and pH and exposure to detergent and washing test. The results obtained 22 isolates of lipolytic bacteria isolated from PTPN V Sei Buatan palm oil mill effluent. Three selected isolates (LSSB 3.4; LSSB 3.3; LSSB 4.1) had lipolytic index of 7.26 (LSSB 3.4), 6.02 (LSSB 3.3) and 5.88 (LSSB 4.1), and lipase activity of 6,75 U/ml (LSSB 3.4), 5.125 U/ml (LSSB 3.3) and 4.125 U/ml (LSSB 4.1). The enzyme remains active at a temperature of 30-70℃, pH 6-11, with exposure to a detergent concentration of 1-5% and can remove oil from cotton fabrics with a percentage of 74.3% (LSSB 3.3); 63.2% (LSSB 3.4); 61.2% (LSSB 4.1). Lipase from the three isolates has the potential to be developed as a biodetergent formulation.
Keywords: Biodetergent, lipase activity, lipolytic bacteria, palm oil mill effluent, washing test

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1803124016

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Biologi., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1803124016

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Deti

Versi lain/terkait

Lampiran Berkas

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