CD Skripsi
Analisis Biodegradasi Plastik Polyethylene Terephthalate (Pet) Oleh Isolat Bakteri Termofilik Dari Sumber Air Panas Padang Ganting, Tanah Datar, Sumatera Barat
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SUMMARY
Polyethylene terephthalate (PET) is a plastic polymer from the polyester group. PET is a polymer composed of terephthalate acid and ethylene glycol as monomers. The PET biodegradation test has been done using isolated thermophilic bacteria from Padang Ganting hot spring called Pseudomonas sp. genus. LBKURCC173, LBKURCC175 and Thermus sp. genus. LBKURCC174, LBKURCC176, LBKURCC177, LBKURCC178. In the biodegradation of plastic process, microorganism will secrete an enzyme that could degrade the polymer to a simple form and uses it as a carbon source. The purpose of this study was to determine the ability of thermophilic bacteria to degrade 1,5 x 1,5 cm "Le Minerale" mineral water bottles added to NA and MSM media at a temperature of 50oC for four weeks of incubation. The result of PET biodegradation was measured by the weight loss, microscope, and SEM analysis, then FTIR. Isolate ability test for four weeks obtained LBKURCC173 bacteria isolate the most degradation PET in NA and MSM media with the percentage loss of PET plastic on NA media 0,47% and MSM media 0,61%. The microscope test analysis did not show any changes in PET plastic surface, while SEM analysis showed LBKURCC173 bacteria on NA and MSM media showed cracks and wavy surface. FTIR analysis showed that there was a shift in the wave number of the C=O, C=C=C and C-O functional groups to a lower direction causes the bond length to increase and the strength of PET to be weak.
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