CD Skripsi
Biosintesis Dan Karakterisasi Nanopartikel Zno Menggunakan Ekstrak Daun Sirih Hijau (Piper Betle L.) Dan Sifat Antibakteri
ABSTRACT
An innovative approach to discovering effective antibacterial and antifungal agents for preventing diseases and extending the shelf life of vegetables and food can be achieved by utilizing nanotechnology through nanoparticle synthesis. The biosynthesis of zinc oxide nanoparticles (ZnO NPs) using various plant extracts has shown promising results that ZnO NPs are safe for human use due to their natural and biodegradable properties. This research aims to synthesize ZnO nanoparticles and analyze the effect of precursor and reducer volume ratios on their physical and antibacterial properties against Escherichia coli and Staphylococcus aureus. The synthesis was carried out using different volumes of green betel leaf (Piper betle L.) extract and zinc nitrate hexahydrate (Zn(NO3)2.6H2O) solution, namely A (1:1), B (1:5), and C (1:10), followed by the addition of 1 M NaOH until each variation reached a pH of 7. The resulting nanoparticles were characterized using UV-Vis spectrophotometry, resulting in an absorbance peak in the wavelength range of 250-330 nm with absorbance intensities of 1.03, 0.54, and 0.24 a.u., respectively. The energy band gap values were in the range of 3.16-3.35 eV. XRD characterization of sample A showed a particle size of 11.9 nm with Miller indices of (100), (002), (101), (103), (112), and (201). SEM characterization showed a spherical morphology and grains with an average size of 436.2 nm. The antibacterial properties against S. aureus and E. coli showed that the higher the concentration of the test solution, the larger the inhibition zone against the bacteria. The most significant inhibition zones for S. aureus and E. coli were 15.02 ± 0.51 and 12.0 ± 0.44 mm at a 0.3 gr/ml test solution concentration, respectively.
Keywords: ZnO nanoparticle, biosynthesis, betel leaf extract, antibacterial properties, Escherichia coli, Staphylococcus aureus
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