CD Skripsi
Uji Metabolit Sekunder Trichoderma virens Sebagai Antifungi Terhadap Colletotrichum capsici secara In-vitro
Chili (Capsicum sp.) is a horticultural plant that has quite complete nutritional content and has high economic value. One of the factors causing problems that are often encountered in chili cultivation is anthracnose disease caused by the fungus Colletotrichum capsici. This study aims to determine the effect of Trichoderma virens secondary metabolites as an antifungal against
C. capsici in-vitro and to obtain the best concentration in inhibiting the growth of the C. capsici fungus. This research was carried out experimentally
using a completely randomized design (CRD) consisting of six treatments and four replications to obtain 24 experimental units. This research consisted of 7
stages, namely: (1) rejuvenation of T. virens fungus isolates, (2) rejuvenation of
C. capsici fungus isolates, (3) production of T. virens culture filtrate,
(4) in-vitro test of T. virens antifungal activity against C. capsici,
(5) preparation of C. capsici suspension, (6) Preparation of red chili fruit,
(7) application of T. virens filtrate to fruit and inoculation of C. capsici to red chili fruit. The results of the analysis of variance were continued with a further Honest Significant Difference (BNJ) test at the 5% level to compare the means between treatments. The results of research on growth inhibition of C. capsici showed that culture filtrate of T. virens was able to inhibit the growth of C. capsici in-vitro, different secondary metabolite concentrations provided inhibition of the growth of C. capsici in vitro, a 40% concentration of T. virens secondary metabolites was able to inhibit the growth of the fungus C. capsici on PDA media with an inhibitory power of 100%, and a secondary metabolite concentration of T. virens of 40% resulted in the initial symptoms appearing and the intensity of fruit rot tending to be the longest, namely 6.40 days and the lowest, namely 11.25%.
Keywords : Colletotrichum capsici, Trichoderma virens, Chili, Secondary
Metabolite
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