Image of Analisis Hubungan Variasi Genetik Gen L1 Dan L2 Human Papillomavirus Tipe 16 Dan 18 Dengan Perubahan Epitop Serta Karakteristik Klinis Pasien Kanker Serviks Di Provinsi Riau
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Analisis Hubungan Variasi Genetik Gen L1 Dan L2 Human Papillomavirus Tipe 16 Dan 18 Dengan Perubahan Epitop Serta Karakteristik Klinis Pasien Kanker Serviks Di Provinsi Riau

Introduction: Cervical cancer is a malignancy of the cervix caused by persistent infection with human papillomavirus (HPV), particularly types 16 and 18. It is the second most common cancer among Indonesian women, with a 56% mortality rate. The L1 and L2 genes encode capsid proteins used to form virus-like particles (VLPs) in HPV vaccines. This study aimed to analyze genetic variations in the L1 and L2 genes of HPV-16 and HPV-18, assess their impact on epitopes, and examine their correlations with clinical features of cervical cancer patients.
Method: DNA was amplified and sequenced from HPV-infected cervical tissue biopsies. A total of 16 sequences of the L1 and L2 genes from HPV-16 and 16 sequences from HPV-18 were analyzed. Genetic variations were identified by comparing sample sequences to NCBI references. Epitope prediction was conducted in silico for B and T lymphocytes, followed by comparison with reference epitopes.
Results: Amino acid mutations in L1 and L2 genes altered epitope profiles. Several novel epitopes were identified with varied binding affinities to B and T lymphocytes (MHC-I and MHC-II). Some had lower affinities than references, while others showed higher affinities, suggesting potential for stronger immune activation. No significant correlation was found between genetic variation and cancer stage or histological grade.
Conclusion: Genetic variations in L1 and L2 genes of HPV-16 and HPV-18 alter the epitopes and may affect immune recognition. No significant association was observed with clinical features. These findings support the development of vaccines tailored to HPV genetic diversity in Indonesia.



Keywords: L1 Gene, L2 Gene, HPV-16, HPV-18, Cervical cancer, HPV vaccine

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Perpustakaan Universitas Riau 2310246498
2310246498
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No. Panggil
2310246498
Penerbit
Pekanbaru : Universitas Riau PASCA Magister Ilmu Biomedis.,
Deskripsi Fisik
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Bahasa
Indonesia
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2310246498
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Tipe Media
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Tipe Pembawa
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Edisi
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Magister Ilmu Biomedis
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Pernyataan Tanggungjawab
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