CD Skripsi

Evaluasi Produksi Senyawa Antibiotik Menggunakan Dua Konsentrasi Spora Jamur Gliocladium Sp. T.N.C73

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Gliocladium sp. T.N.C73 was isolated because it has the ability to produce chitinase. Recent indications that produce several secondary metabolites that antibiotic are soluble in ethyl acetate and methanol. Antibiotic compounds which were suspected to be peptaibols. In this study aimed to compare the antibacterial activity of two concentrations of spores Gliocladium sp. T.N.C73. Antibacterial production with fermentation in liquid medium and spore volume variation is 2,5x108 spores or 2x1011 spores / for 50 mL media. Until 14 days in media antibiotics and extracted with ethyl acetate. Antibiotics in the ethyl acetate extract are evaporated and the residue dissolved in methanol. Furthermore peptaibol presence detection is done by using Thin Layer Chromatography (TLC) with eluent diclorometan (DCM) : methanol (80:20) and the stain was observed after spraying reagent 0.5% p-anisaldehyde and heated at 110oC for 20 minutes. Antibacterial test by disc diffusion method with a concentration of 10 L/discs. Antimicrobial test against Bacillus subtilis and Erwinia carotovora subsp. carotovora. TLC result with the addition of 0,5% p-anisaldehyde indicate a presence on the stain peptaibol P1 (Rf 0.92) and P5 (Rf 0.92) which show the red spot. The test results showed that the antibacterial antibiotics produced extract A has a greater inhibition of the extract B. In B.subtilis test, is extract A = (19.1 ± 0.2) mm, extract B = (10.6 ± 0.6) mm, and a positive control = (10.2 ± 0.2) mm. The inhibition of E.caratovora subsp. carotovora, is extract A = (19.5 ± 0.1) mm, extract B = (7.9 ± 0.2) mm, and a positive control = (10 ± 0.1) mm and no inhibition against any negative control test bacteria.
Key words : Gliocladium sp. T.N.C73, peptaibol, antibacterial.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02.013 (0063)

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Kima., 2013

Deskripsi Fisik

xiv, 35 hlm.;ill.; 29 cm

Bahasa

ISBN/ISSN

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Klasifikasi

03 02.013 (0063)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

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Info Detail Spesifik

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Pernyataan Tanggungjawab

Dewa

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