Pemanfaatan Ekstrak Daun Kersen (Muntingia Calabura L) Untuk Menghambat Pertumbuhan Bakteri Edwardsiella Tarda

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The research was carried out at the Faculty of Mathematics and Natural Science, University of Riau, in the Chemical Laboratory of the Faculty of Mathematics, Natural Science, and Health, Muhammadiyah University, Riau, and Fish Parasite and Disease Laboratory of the Faculty of Fishery and Marine, University of Riau, from July until October, 2018. The objective of the research was to find out bioactive or anti-bacterium contained in the extract of cherry leaf (Muntingia calabura), its sensitivity which inhibited the growth of Edwardsiella tarda bacteria, the dosage of Minimum Inhibitory Concentration (MIC) extract of cherry leaf which could inhibit the growth of E. tarda bacteria and lethal dosage50 of its extract on lele dumbo (Clarias gariepinus), a kind of catfish, using soaking method.
The research used KIRBY-BAUER disc with blank disc in 6 mm diameter, repeated 3 times in order to decrease errors. The testing material was cherry leaves fund in the surrounding University of Riau and lele dumbo 120 fishes. Toxicity LD50 was analyzed by using Reed and Muench method in Harmita and Radji (2008). The treatment was carried out by giving cherry leaf extract with the dosage of 10,000 ppm, 9,000 ppm, 8,000 ppm, 7,000 ppm, 6,000 ppm, 5,000 ppm, 4,000 ppm, 3,000 ppm, 2,000 ppm, and 1,000 ppm; Novobiocin was used as the control for sensitivity test and MIC test dosage of 800 ppm, 750 ppm, 700 ppm, 650 ppm, while LD50 test was 800 ppm, 750 ppm, and 700 ppm.
The result of the research showed that the extract of cherry leaf contained compounds which could be identified in phytochemicals test which consisted of terpenoid/steroid, flavonoid, phenolic, and saponin. The result of thin-layer chromatography test with the values of Rf terpenoid/steroid 0.88, flavonoid 0.6 and phenolic 0.6, cherry leaf extract was sensitive toward E. tarda bacteria and could inhibit the growth of E. tarda bacteria the dosage of 700 ppm at the average inhibit zone of 6.14 mm. Minimum Inhibitory Concentration (MIC) of cherry leaf extract was 700 ppm with the average of the number of colonies of 261.66 CFU/mL. The result of toxicity LD50 test of cherry leaf extract on lele dumbo (Clarias gariepinus), using soaking method was at the dosage of 720.06 ppm.

Keywords: Cherry Leaf Extract, Edwardsiella tarda, Anti-bacteria, LD50

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 16. 219 (0012)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Kelautan., 2019

Deskripsi Fisik

xv, 88 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 16. 219 (0012)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

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