CD Skripsi
Pertumbuhan Bakteri Bacillus Cereus Pada Media Cair Dengan Sumber Protein Yang Berbeda
The aim of this research was to determine the optimal media composition
and incubation time for the growth of Bacillus cereus and B. cereus isolate
consortium with other strains isolate. Using an experimental method, which was
testing 2 groups of bacterial isolates; B. cereus strain SN7 (isolate N) and bacterial
consortium (combination of 5 isolates of B. cereus with different strains). Each
group of isolates was grown in sago waste media added with two different protein
sources (egg whites and skim milk) and 3 different concentrations in each protein
source (8%, 10%, and 12%) with 3 replications in each treatment. Bacterial
culture was measured every 6 hours for 24 hours using three methods; total agar
plate count, spectrophotometric methods, and bacterial cell biomass methods.
There was no significant difference between the growth of these two groups of
bacterial isolates. Optimal growth of each isolate was in the sago waste media
which was added with egg white but with different concentrations, namely 10% in
B. cereus SN7 and 12% in the consortium. B. cereus consortium showed higher
growth than B. cereus SN7. The optimal time for bacteria to grow was in the
range of 12 to 24 hours.
Keywords: Bacillus cereus, optimal growth, protein, media, incubation time.
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