CD Tesis
Pengaruh Perbedaan Konsentrasi Limbah Cair Kelapa Sawit Terhadap Pertumbuhan Dan Komposisi Kimia Chlorella Sp.
Palm oil industry waste is rich in nitrate and phosphate content and it be
used as nutrient resources for Chlorella culture. By culturing the algae, the
nitrate and phosphate content may be used for growing and thus their
concentration in the waste is reduced and the waste quality is improved. To
understand the effects of palm oil industry liquid waste concentration toward
growth and chemical composition of Chlorella sp, a study was conducted in April
– May 2019. A CRD was applied, 5 treatments and 3 replications. The waste was
mixed with distilled water and there were 5 waste concentrations applied, namely
P1 (0%), P2 (15%), P3 (20%), P4 (25%) and P5 (30%). The algae population
was counted every day for 24 days and the biomass was measured during the
exponential phase (in the 12nd day). Water quality parameters measured were
temperature, pH, dissolved oxygen, free carbon dioxide, nitrate, phosphate and
chlorophyll-a content.
Results shown that the peak of algae population as well as the highest
biomass was achieved in the 12nd day, they were 4.64 x 106 cells/mL in 30%
concentration of waste and 0.77 g/L in 25% respectively. The highest protein
content was in 25% (45%), while the highest fat and carbohydrate content,
however, was in 0% (12.05% and 24%).
In this research, it is clear that Chlorella sp. is able to use nitrate and
phosphate content in the waste for growing and thus improving the waste quality.
The best result was obtained in 30%. During 12 days of experiment, nitrate
reduced from 14.341 mg/L to 5.279 mg/L, and phosphate reduced from 1.817
mg/L to 0.872 mg/L, while chlorophyll-a peaked in 0.403 mg/L. During the
experiment, 30% shown the best improvement in DO (from 8.08 to 9.94 mg/L)
and CO2 (from 7.06 mg/L to 2.75mg/L). In all treatments applied, the water
quality was relatively good, with temperature 280C -300C and pH 6.33 – 9.50.
Keywords: Palm oil liquid waste, chlorophyll-a, nitrate, phosphate
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