CD Skripsi

Kemampuan Biodegradasi Bakteri Proteolitik Pada Ekosistem Mangrove

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The purpose of this study was to an awareness the optimal time for the growth of
proteolytic bacteria in producing the protease enzyme and determine the effectiveness of
the protease enzyme from proteolytic bacteria in degrading mangrove litter. The method
used was experimental method by using two proteolytic bacteria isolates, namely Bacillus
manliponensis (K6) and Bacillus toyonensis (K20). This method used one treatment for
each proteolytic bacterial isolate, namely the addition of different extract containing
protease enzyme (0%, 25%, and 50%) with three replications. Measurement of bacterial
growth was carried out every 6 hours for 24 hours using Total Plate Count (TPC) and
spectrophotometric method. B. manliponensis and B. toyonensis have enzyme activity as
indicate by the presence of a clear zone on Zobell Marine Agar 2216 and skim milk 1%.
The growth bacteria based on the TPC measurement was directly proportional to the
spectrophotometric measurement results. The highest bacterial count was (1.39 x 108
CFU's/ml) K6 bacteria and (1.52 x 108 CFU's/ml) K20 bacteria. The results of the
measurement of cell growth in the spectrophotometric method occurred at the 6th hour,
namely 10.36 x 108 CFU's/ml (K6 bacteria) and 10.97 x 108 CFU's/ml (K20 bacteria).
The optimum time of the protease enzyme occurred at 6 hours, which was 0.0258 mg/ml
(K6 bacteria) and 0.0262 mg/ml (K20 bacteria). The highest dissolved protein content
was obtained at the 50% dose of 0.054 mg/ml (K6 bacteria) and 0.055 mg/ml (K20
bacteria), while the lowest was at 0%, namely 0.050 mg/ml (K6 bacteria) and 0.051
mg/ml (K20 bacteria).
Keywords: Biodegradation, Growth, Proteolytic Bacteria, Protease Enzyme, Dissolved
Protein
1) Student of the Faculty of Marine and Fisheries, Riau University
2) Lecturer of the Faculty of Marine and Fisheries, Riau Universty

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

04 03. 120 (0091)

Penerbit

Pekanbaru : Universitas Riau – Fakultas Perikanan Dan Ilmu Kelautan – Ilmu Kelautan., 2020

Deskripsi Fisik

xii, 29 hlm.; ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

04 03. 120 (0091)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Daus

Versi lain/terkait

Lampiran Berkas

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