CD Tesis

Potensi Metabolit Sekunder Bakteri Heterotrofik Laut Penghambat Bakteri Patogen

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Marine heterotrophic bacteria are microorganisms that can produce antimicrobial
compounds. The purpose of this study is to isolate and identify heterotrophic types
of bacteria that are antagonistic towards pathogenic bacteria on marine fish, analyze
secondary metabolite extracts of heterotrophic bacteria towards pathogenic bacteria
on marine fish, and analyze the metabolism of heterotrophic bacteria towards
pathogenic bacteria on marine fish. The method used in this research were Gram
test, biochemistry test, diffusion agar, 16S rDNA, extraction, phytochemical test,
column flash chromatography, thin layer chromatography, recrystallization, high
performance liquid chromatography, spectroscopy UV, spectroscopy IR,
spectroscopy NMR. The results showed that 8 terotrophic bacterial isolates were
circular, concentric and irregular. Creamy and white colors, smooth and curved
edges, flat and convex. The inhibitory antagonistic test of isolates JS6, JS11 and
JS22 on the growth of Vibrio alginolyticus bacteria were classified as moderate.
JS19 isolates on the growth of Aeromonas hydrophila bacteria were classified as
moderate. JS4 isolates on the growth of Pseudomonas aeruginosa bacteria were
moderate and the other isolates were classified as weak. The results of BLAST
system searching for isolates JS4, JS11 and JS25 have similarity in DNA with
Bacillus sp. Isolate JS6, JS10 and JS22 with Bacillus cereus. JS8 isolates with
Bacillus toyonensis and JS19 isolates with Pseudoalteromonas sp. Phytochemical
test of eight heterotrophic bacterial isolates produced terpenoid class compounds.
The inhibition test of Bacillus sp. strain JS4 secondary metabolite extract has the
highest inhibitory power on the growth of pathogenic bacteria V. alginolyticus (17.5
mm), A. hydrophila (16.8 mm) and P. aeruginosa (17.3 mm). 3) NMR spectrum
analysis, be identified of compound JS4-E-01 is N- [2- (3-hydroxypropanoyl)
ethenyl] 3-hydroxypropanamide. The combined inhibitory power of JS4-E-01
towards V. alginolyticus bacteria was 13.3 mm, towards A. hydrophila was 12.3
mm and towards P. aeruginosa 10.5. Conclusion, marine heterotrophic bacteria
from the waters of Pakning River, Bengkalis Regency, Riau Province, can produce
antibacterial compounds that can inhibit the growth of pathogenic bacteria on fish.
Keywords: Heterotrophic bacteria, secondary metabolites, antibacterial,
pathogenic bacteria, Bacillus sp.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 03. 321 (0001)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Disertasi Ilmu Kelautan., 2021

Deskripsi Fisik

xvi, 131 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 03. 321 (0001)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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