CD Tesis
Potensi Minyak Ikan Patin (Pangasius Hypopthalmus) Dengan Minyak Hati Ikan Hiu Sebagai Pangan Fungsional
Catfish is the main commodity in Riau Province which has a high fat content. Shark is a type of sea water fish that is often encountered as a byproduct, sharks have a high fat content, therefore i am interested in studying the potential of fish oil from the by-product of catfish with shark liver oil, with this combination it is hoped that it can increase the content of omega 3, omega 6, and omega 9 in fish oil.
The research was carried out from October- December 2020 to completion in the Laboratory of Fisheries Product Processing and Fisheries Product Chemistry, Faculty of Fisheries, Riau University, and the BBIA Laboratory, Integrated IPB, and the Natural and Mineral Engineering Technology Laboratory of Riau University. This study aims: 1). to obtain chemical characteristics (free fatty acid content, acid number, peroxide and soaping) crude catfish oil. 2). characteristics data (free fatty acid content, acid number, peroxide number and saponification number in pure catfish oil and shark liver oil. 3). data on the physical and chemical characteristics of the combination of catfish oil and shark liver oil. 4) information on the potential of the combination of catfish oil and shark liver oil as a functional food.
This study used an experimental method which was designed to consist of 3 stages, stage 1) was the extraction of catfish oil. Stage 2) purification of catfish oil and characterization of physicochemical properties of catfish oil, bottle shark liver oil. Stage 3) is to study and characteristic the physicochemical properties of the combined combination of catfish oil and the resulting bottle shark. The analysis parameters were consisted of chemical characteristics (free fatty acid number, peroxide, saponification, acid number, iodine, total oxidation, p-anisidin, and fatty acid profile), physical characteristics (viscosity and clarity) and analysis of antioxidant activity
The results of the analysis of catfish oil with free fatty acids were crude fish oil 1.93% to 1.34% in pure oil. The safonification number of crude oil 177.02 mg KOH/g becomes 191.92 mg KOH/g after purification. The peroxide number of crude catfish oil was 8.52 meq / kg to 4.38 meq / kg after purification. The free fatty acid value contained in shark liver oil is 0.58%, the acid number value is 1.16 mg KOH / g. the saponation number is 181.66 mg KOH / g. The peroxide number contained in shark liver oil was 21.84 meq / kg.
The physico-chemical characteristics of catfish oil in combination with shark liver oil clarity value of mixed fish oil in AI treatment. 71.75%, A2. 71.35% A3. 71.85%. The value of density and viscosity in mixed fish oil in treatment A1. 0.681 g / mL and 24.44cPs, A2. 0.683 g / mL and 25.28 c.Ps, A3. 0.685 g / mL and 23.90 cPs. The free fatty acids contained in the A1 treatment were 1.59%, A2 2.19%, A3 1.51% with the acid number A1. 2.86 mg KOH/g, A2. 4.36 mg KOH/g, A3. 3.00 mg KOH/g. the soaping number of A1. 158.66 mg KOH / g A2. 142.21 mg KOH/g, A3. 162.28. Anisidine values in the treatment of A1.24.16 meq / kg, A2.21.53 meq / kg and A3. 22.79 meq / kg. The TOTOX value contained in the
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A1.55.16 meq / kg, A2 treatment. 53.33 meq / kg and A3. 59.43 meq / kg. Iodine A1 value. (83.97 gI2/ 100g) A2. (63.46gI2/ 100g) and A3. (84.82 gI2 /100g).
The highest SAFA content was in sample A3 (26.82%) and the lowest SAFA content was in sample A2, namely (23.63%). The highest MUFA was found in sample A3 (31.71%) and the lowest MUFA was found in sample A2 (29.97). The highest PUFA content was in sample A2 (32.46%) and the lowest PUFA content was in sample A3 (27.80%). The highest EPA content was found in sample A3 (0.13%) and the lowest EPA content was A2. (0.09%). The highest DHA content was in sample A3 (0.25%) and the lowest was in sample A1 (0.09%). Anti-oxidants in mixed fish oil were reduced in the A3 treatment (107.29), while the highest was in the A1 treatment with an average value (236.76), the lower the IC50 value, the higher the antioxidant levels in carrying out free radical absorption.
The free fatty acid content, acid number, peroxide number and saponification number of crude catfish oil, pure catfish oil and pure shark liver oil have met the standard (IFOMA 1998). The combination of catfish oil and shark liver oil based on the test results of primary and secondary oxidation parameters such as free fatty acids, acid numbers, peroxide numbers, saponification numbers, iodine numbers, p-anisidin and totox values have met the standards (IFOMA 1998) but still not meets the standards (IFOS 2014). Physical analysis such as clarity, density and viscosity of the combination of catfish oil with shark liver oil has met the standards of (IFOMA 1998),
The combination of catfish oil with shark liver oil also contains SAFA, MUFA and PUFA. The highest EPA and DHA content in the combination treatment of 2 shark liver oils: 1 catfish oil in a combination unit of mL. The combination of catfish oil also has potential as an antioxidant with IC50 values 107-236.
Keywords: Physochemical, Fatty Acids, DHA, EPA, Antioxidants
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