CD Tesis
Aspek Biologi Ikan Patin (Pangasianodon Hypophthalmus) Yang Dipelihara Dengan Efektivitas Penerapan Sistem Bioflok Pada Manipulasi Fotoperiod
The effectiveness of catfish rearing can be improved by applying the combination of biofloc system and photoperiod manipulation, where the fish was reared under dark condition. Photoperiod manipulation is useful for improving the growth of catfish, while the biofloc system functions was to maintain the water quality. This study aims to analyze the health status of catfish, the condition of the catfish tissue structure and to determine the water quality after being treated with the biofloc system and photoperiod manipulation. While the benefits obtained from this study was to provide the maintenance technique of catfish (P. hypophthalmus) culture, as the fish may grow faster, provide cheaper operational fee, environmental friendly and produce fish that is for consumption. This research was conducted in June-September 2020 at the Aquatic Biology Laboratory and Terpadu Laboratory of Riau University.
This study used a randomized block design with 3 photoperiod groups, 3 biofloc treatments and 3 replications. The groups used in this study were 18 hours dark (18D), 24 hours dark (24D), and Natural groups. The biofloc used was derived from EM4 and added molasses. Catfish are kept in a 110 liter capacity container filled with 100 liters of water as many as 18 units with each pond containing 30 fish. Sampling for fish growth was carried out every week, where as many as 3 fish/treatment for nine weeks. Blood sampling and histology were taken twice, at the beginning and at the end of the study. Water quality sampling was carried out three times, namely at the beginning, middle and end of the study. The parameters observed were length of weight, condition factor, blood condition, tissue structure (gills, liver, kidneys, and intestines) and water quality such as temperature using a thermometer, pH using a pH indicator, BOD5 and DO using titration, while nitrate, phosphate and ammonia was measured using spectrophotometry based on APHA (2012).
Results shown that length and weight of catfish reared in each treatments, with a biofloc system and photoperiod manipulation did not show any significant difference. The best results on the length and absolute body weight of catfish were found in the 24D without biofloc treatment which was 19.27 cm TL and 74.06 g BW, then followed by natural with floc treatment (19.24 cm TL and 57.89 g BW), then 24D with floc system (19.11 cm TL and 60.40 g BW), in 18D without floc treatment (18.77 cm TL and 72.75 g BW), 18D with floc treatment (18.14 cm TL and 51.56 g BW) and the lowest was in natural photoperiod without floc treatment (17.73 cm TL and 58.61 g BW). The ANOVA results did not show any
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significant differences in each treatment group. Results of blood condition study shown that the treated fish was normal, no damage nor major abnormalities found in the blood all fish treated. In the tissue structure of the fish, however, abnormalities were found in the gill, such as hyperplasia, hypertrophy, and necrosis. In the gill of fish, especially the fish reared in the biofloc system, parasite Dactylogyrus sp. was found and it is predicted that the abormalities in the gill structure were caused by that parasite. The structure of liver, kidneys and intestines are in normal. The water quality in the rearing tank treated with biofloc system provided a better results, as it has lower BOD5, ammonia, nitrate and phosphate concentration. However, water quality in all treated rearing tanks shown normal condition and the fish was able to live in those tanks. Based on the data obtained, it was concluded that the combination of the biofloc system and photoperiod manipulation had a positive effect on the growth of catfish, but did not negatively affect the blood picture and tissue structure of catfish.
Keywords: Biofloc, Controlled Photoperiod, Fish Health, Fish Histology, Nocturnal Fish
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