CD Skripsi

Karakteristik Bakteri Tanah Gambut Hutan Alami Cagar Biosfer Giam Siak Kecil Bukit Batu Diisolasi Dengan Media Antibiotik

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Bacterial isolates named LBKURCC419, LBKURCC423 and LBKURCC424 are
natural forest soil bacteria isolated using tetracycline and amoxicillin media from
the Giam Siak Kecil Bukit Batu Biosphere Reserve, Riau Province. Bacteria from
the natural environment need to be characterized to determine the species, the
level of resistance to tetracyclines and amoxicillin, the gene coding for resistance
to know how the pattern of resistance spread in the natural environment. In this
research, the bacterial isolates were identified to determine the bacterial species
and genes encoding tetracycline resistance using molecular biology techniques as
well as minimum inhibitor concentration (MIC) value was also determined by
microdiffusion to determine the level of resistance of bacteria to tetracyclines and
amoxicillin. The bacterial chromosomal DNA was isolated using the TIANamp
DNA bacteria kit, then continued to gene 16S rDNA amplification (PCR method)
and a sequencing process was carried out to determine the type of bacterial
species. The results of DNA sequence analysis were aligned with Bioedit and
analyzed with BLAST and MEGA X. The results of BLAST and phylogenetic
tree analysis showed that the three species of isolates were 99% identical to some
Bacillus. LBKURCC419 isolate has similarities with Bacillus siamensis, while
LBKURCC423 and LBKURCC424 isolates are the same bacteria and have
similarities to Bacillus nitratiraducens. The eight tet genes were identified
negative that meant the isolates did not have those genes. The MIC values of
LBKURCC419, LBKURCC423 and LBKURCC424 isolates were 0.75, 3 and 3
μg/ml respectively, meanwhile the MIC values for amoxicillin were 12, 12 and 8
μg / ml. According to CLSI 2015, the resistance level of the three isolates with
MIC values ≤ 4 mg / ml are classified as susceptible to tetracyclines and resistant
to amoxicillin. Based on the data obtained, it can be concluded that the three
natural forest bacteria are Bacillus bacteria which are susceptible to tetracycline
and resistant to amoxicillin.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 121 (0037)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2021

Deskripsi Fisik

x, 47 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 121 (0037)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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