Abstract This study aims to determine the effect of different papain enzyme concentrations on collagen extraction from the skin of catfish (Pangasius sp.). The method used was an experimental method with completely randomized concoctions (CRD). The treatments given consisted of E0 (Control), E1 (5% papain enzyme), E2 (10% papain enzyme), E3 (15% papain enzyme). This research consisted of 3…