Abstract The tofu industrial waste is rich in organic materials and it may be used as nutrient sources in the Spirulina sp culture. A study aims to understand the effect of EM4 fermented tofu liquid waste addition in the media toward abundance and biomass of Spirulina sp has been conducted in February to March 2019. The fermented waste was made by mixing 35 L of tofu waste and 35 mL of EM4…