Abstract Pangasianodon hypopthalmus can be reared under biofloc system and short photoperiod. To understand the effect of biofloc system and photoperiod manipulation towards blood structure of P. hypopthalmus a study was conducted in September to December 2020. This experiment uses a factorial CRD design with 2 levels for B factor (biofloc and no biofloc), 3 levels for P factor (Natural, 1…