CD Skripsi

Deteksi gen ß – globin dari apusan glans penis pada pria pasangan pasien kanker serviks

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Human papillomavirus (HPV) is a virus that spreads skin-to-skin which will cause pre-cancerous lesions or cancerous lesions. HPV infection in men are often asymptomatic, so that they can increase the risks of cancer in their partners. So it is necessary to have screening to prevent HPV infection in male. This study aims to determine the optimization of the sampling used for the detection of HPV in men. Sampling optimization can be done by detecting the β-globin gene. This was descriptive study for the period October 2021 – February 2022 with a total sample of 12. The sample used was a swab from the male glans penis of a partner with cervical cancer. The sample was then isolated using an extraction method with the principles of Salting Out, Silica Column, and Magnetic Beads. The samples were then analyzed with Polymerase Chain Reaction (PCR).
The results showed that the extraction method for detecting the β-globin gene was the silica column and magnetic bead extraction principle method. PCR annealing conditions and primer concentration of β-globin gene further optimization because there are nonspecific bands. The percentage of samples that were positive for the β-globin gene in this study was 100% so that sample optimum to detecting HPV in male.
Keywords : HPV, Glans Penis, PCR, β-globin gene.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

08 01.122 (0020)

Penerbit

Pekanbaru : Universitas Riau – Kedokteran – Pendidikan Dokter., 2022

Deskripsi Fisik

xiv, 50 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

08 01.122 (0020)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN DOKTER

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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