CD Skripsi
Pengaruh Ph Berbeda Terhadap Hidrolisat Protein Belut (Monopterus Albus) Menggunakan Enzim Papain
ABSTRACT
Eel (Monopterus albus) is one of the raw materials for protein hydrolyzate. Eel
(M. albus) is known to have a complete amino acid composition. Protein
hydrolyzate is made using enzymes. One of the factors that affect the
characteristics of the protein hydrolyzate produced is pH. This study was aimed
to determine the effect of pH on protein hydrolysis of eel (Monepterus albus)
using papain enzyme. The research method used was an experimental method
with a non-factorial completely randomized design with pH treatment consisting
of P1 (pH 6.5), P2 (pH 7), P3 (pH 7.5), and 3 repetitions. Parameters analyzed
consisted of yield, dissolved protein, degree of hydrolysis, and amino acid
composition. The results showed that the yield of the hydrolyzate was treated
with pH 6.5 (Pi), 7 (P2), and 7.5 (P3) 19.09 ± 0.16, 21.28 ± 0.30, and 22, 26 ±
0.28 (P3), respectively. The results of the analysis of dissolved protein and the
degree of hydrolysis of eel protein hydrolyzate with different pH treatments
resulted in 1.61 ± 0.07 (P1), 2.55 ± 0.17 (P2), 3.17 ± 0.0(P3), and the degree of
hydrolysis. of 0.27 ± 0.03 (P1), 0.38 ± 0.004 (P3), 0.47 ± 0.28(P3). The highest
composition of essential amino acids and non-essential amino acids were lysine
(0.258%) and glutamic acid (1.522%). The best hydrolyzate was produced in P3
treatment (pH 7.5) with higher yield, degree of hydrolysis and dissolved protein
from other treatments.
Keyword: amino acid, degre of hydrolysis, pH. yield
1) Student at Faculty of Fisheries and Marine Science, Universitas Riau
2)Lecturer at Faculty of Fisheries and Marine Science, Universitas Riau
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