CD Skripsi

Deteksi Gen Β-Globin Dari Apusan Batang Penis Pada Pria Pasangan Pasien Kanker Serviks

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HPV infection in men shows a lack of visible penile lesions, this has led to
the need to seek more sensitive and specific diagnostic methods for detecting HPV
in men. A trusted diagnostic approach for men is HPV DNA testing. To ensure
appropriate sampling, the presence of human DNA from the smear was assessed
by PCR detection of the β-globin gene, the success of the formation of the PCR
product can be influenced by several factors such as the choice of DNA extraction
method, primer selection, temperature during the annealing stage to the number
of cycles in one reaction during the amplification process. . This study aims to
determine the extraction method, annealing temperature, optimal primer
concentration for the detection of β-globin genes and the percentage of -globin
genes that can be detected from penile smear samples in male partners of cervical
cancer patients at Arifin Achmad Hospital, Riau Province. This research is a
descriptive research conducted in the integrated biomedical laboratory of FK
UNRI. The extraction method was optimized by comparing 3 extraction methods
(Manual, Column, and Automatic) while the optimized pcr conditions included
annealing temperature (50°C;55°C), primary concentration (0.1μM; 0.2μM;
0.3μM) b-globin gene (primaryβ -globin-F 5'-GAAGAGCCAAGGACAGGTAC-3'
and Primary β-globin-R 5'-CAACTTCATCCACGTTCACC-3'). The results of this
study indicate that the automatic method is the optimum extraction method, and
for PCR optimization the annealing temperature of 55°C, and the primer
concentration of 0.2 M is the optimum result obtained.
Keywords: HPV, DNA isolation, PCR optimization, β-globin gene.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

08 01.122 (0027)

Penerbit

Pekanbaru : Universitas Riau – Kedokteran – Pendidikan Dokter., 2022

Deskripsi Fisik

xvi, 55 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

08 01.122 (0027)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN DOKTER

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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