CD Skripsi

Penentuan Ph Dan Temperatur Kerja Optimum Enzim Lakase Trichoderma Asperellum Lbkurcc1 Hasil Fraksinasi Pengendapan 22% Ammonium Sulfat Jenuh

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Laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) belongs to a group of enzymes called blue copper proteins which are capable of oxidizing aromatic and non-aromatic compounds. The laccase enzyme can be produced by fungi. One of the fungi is Trichoderma asperellum LBKURCC1, that was isolated from the rhizosphere of cocoa plantations. Laccase activity is influenced by several factors, including pH and temperature. The aim of this research was to determine the optimum pH and temperature of laccase activity in the 0-22% ammonium sulfate precipitate fraction. In this research laccase was produced by growing T. asperellum LBKURCC1 on rice straw by Solid State Fermentation (SSF). Crude laccase was extracted from the SSF media and semipurified. The purification process was carried out in several steps, that was, protein 22% ammonium sulfate precipitation from a laccase crude extract, separation of the precipitate from the soluble crude extract, solvation of the 22% ammonium sulfate salt precipitate in a sodium acetate pH 5,5 buffer, and dialysis of the solute with an ultrafiltration membrane of 50,000 Dalton Molecular Weight Cut Of (MWCO). The highest laccase activity of the 22% ammonium sulfate fraction on ABTS was found to be at optimum pH of 4,5 and an optimum temperature of 40C, giving a laccase activity of (104,63  8,02) and (145,1  15,1) U/L respectively

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1703110274

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1703110274

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

nadya

Versi lain/terkait

Lampiran Berkas

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