CD Skripsi

Karakteristik Bakteri Patogen Dari Isolat Klinis Rumah Sakit Secara Mikrobiologi Dan Biologi Molekuler

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The bacteria in this study were clinical isolates from hospital patients, which consisted of 3 pus isolates (isolate 3782,3887 and 3901) and 1 urinary isolate (isolate 3936). Bacteria from clinical isolates need to be identified to determine the genus, species, pattern of resistance to antibiotics and resistance genes that play a role in the resistance mechanism. In this study, bacterial isolates were identified by Gram staining and biochemical tests to determine the genus of the isolates. Antibiotic sensitivity test was also carried out using the disc diffusion method. The research continued with the isolation of chromosomal DNA and plasmids. Then proceed with 16S rRNA gene amplification (PCR method) and two-direction sequencing. The results of DNA sequencing were aligned using BioEdit and analyzed by BLAST and MEGA X. Resistance genes were detected by PCR method. The results of microbiological identification showed that in the pus specimen, 1 isolate of Staphylococcus sp. (isolate 3782) and 2 isolates of Coagulase Negative Staphylococcus (isolate 3887 and 3901) has been found, while in urine found 1 isolate of Pseudomonas sp. (isolate 3936). All bacterial isolates were resistant to 10/25 antibiotics and sensitive to 5/25 antibiotics. The results of molecular biology identification stated that isolates 3782 had no similarity to antibiotic resistance sequences in the database, isolates 3887 had 98,52% similarity to Staphylococcus edaphicus, isolates 3901 had 95,79% similarity to Acinetobacter septicus and isolates 3936 had 97,91% similarity to Microbacterium resistens. Of the 4 clindamycin resistance genes (ermA, ermB, ermC, msrA) and 3 cefazolin genes (blaSHV, blaTEM-1, blaOXA-1) tested, the msrA gene was present in the genome and plasmid of all isolates. The genome of isolates 3901 had ermC, blaSHV and blaTEM-1 genes, while isolates 3782 had blaTEM-1 genes. In the plasmid, the blaTEM-1 gene was detected in isolates 3936. Based on the data, all bacterial isolates were resistant to clindamycin and cefazolin had msrA genes in their genome and plasmid. However, not all bacterial isolates had the cefazolin resistance genes. Isolates 3901 had the most number of resistance genes among bacterial isolates.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1803110576

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1803110576

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

nadya

Versi lain/terkait

Lampiran Berkas

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