CD Skripsi

Efek Kultur Hipoksia Terhadap Sekresi Vascular Endothelial Growth Factor Dan Hepatocyte Growth Factor Dari Sel Punca Mesenkimal

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ABSTRACT
EFFECTS OF HIPOXIA CULTURE ON VASCULAR ENDOTHELIAL
GROWTH FACTOR AND HEPATOCYTE GROWTH FACTOR FROM
MESENCHYMAL STEM CELL
By
Agnes Ivana G
Mesenchymal stem cells (MSCs) are present in various tissues such as brain, liver,
bone marrow, adipose tissue, skin, placenta, and umbilical cord and can be
potentially developed as therapies for various diseases. Oxygen concentration
during MSC culture is suggested to affect the function of MSC including the ability
to secrete many biological factors. This study aimed to determine the effects of
hypoxic culture on the levels of vascular endothelial growth factor (VEGF) and
hepatocyte growth factor (HGF) secreted by MSC which were isolated from
umbilical cord. Mesenchymal stem cells were obtained from umbilical cord and
isolated by explant method. Hypoxia was induced using CoCl2 at a concentration
of 100 μM for 24 hours. The secretion of VEGF and HGF in the culture medium
was measured by ELISA method. Differences in the mean of VEGF and HGF levels
in MSC cultured medium between normoxia and hypoxia were analysed by
independent t-test. The results showed that the average VEGF concentration in
normoxic conditions was 0.55 ± 2.45 pg/mL and hypoxic conditions was 28.07 ±
9.49 pg/mL and this difference was statistically significant (p = 0.00). Furthermore,
the average HGF levels in normoxic conditions was 18.00 ± 8.80 pg/mL and
hypoxic conditions was 1.70 ± 9.76 pg/mL and this difference was also statistically
significant (p = 0.013) In summary, this study showed that hypoxia in MSC isolated
from the umbilical cord caused an increase in the secretion of VEGF and
conversely decreased the secretion of HGF.
Keywords: CoCl2, HGF, hypoxia, normoxia, mesenchymal stem cell, VEGF

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1908112645

Penerbit

Pekanbaru : Universitas Riau – Fakultas KEDOKTERAN - PENDIDIKAN KEDOKTERAN., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1908112645

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KEDOKTERAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

tethi

Versi lain/terkait

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