CD Skripsi

Aktivitas Antioksidan Dan Inhibitor Alfa Glukosidase Dari Fraksi Berbeda Rumput Laut Cokelat(Sargassum Sp.)

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ABSTRACT
Sargassum sp. is one of the brown seaweed that contains bioactive compounds, which has antioxidant and alpha glucosidase inhibitor activity. This research aimed to determine the yield, secondary metabolic, total phenolic, antioxidant, and alpha glucosidase inhibitor from the extract and fractions of Sargassum sp. The research method used was an experiment by extracting Sargassum sp with methanol and the extract were fractionated with different polarity solvents (n-hexane, ethyl acetate, and ethanol). Data were analyzed descriptively. The analysis parameters consisted of rendemen, secondary metabolic (qualitative), total phenolics, antioxidants (DPPH and FRAP), and alpha glucosidase inhibitors. The results showed that the yield of n-hexane, ethyl acetate, and ethanol fractions were 4.15, 5.81, and 4.94%, respectively.The secondary metabolic analysis of methanol extract was alkaloids, steroids/terpenoids, saponins, and phenolic. The n-hexane fraction was steroids/terpenoids, saponins, and phenolic. The ethyl acetate fraction was alkaloids, steroids/terpenoids, saponins, and phenolic. The ethanol and water fractions contained only saponins. The total phenolic content of the methanol extract, n-hexane, ethyl acetate, and etanol fractions were 21161.03, 7854.46, 1626.89, and 80.17 mg/kg. Antioxidants in methanol extract, n-hexane, ethyl acetate, ethanol fractions using DPPH were 329.42, 466.25, 535.08, and 80.17 mg/kg. Antioxidants in methanol extract, n-hexane, ethyl acetate, and ethanol fractions using FRAP were 196.15, 294.88, 364.26, and 492.68 mg/kg. Alpha glucosidase inhibitor in n-hexane fraction with an IC50 value of 538110,9 ppm. Extract and fractions of Sargassum sp. with different polarity solvents contained different secondary metabolic. Saponins was found in all solvents, phenolics and steroids/terpenoids were found in methanol extract, n-hexane, and ethyl acetate fractions, alkaloids were found in methanol exract and ethyl acetate fraction. The n-hexane fraction showed the highest activity in total phenol, antioxidant, and alpha glucosidase inhibitor.
Keywords: extract, fraction,total phenol,n-hexane, secondary metabolic
1) Student of the Fisheries and Marine Science Faculty, Universitas Riau
2) Lecturer of the Fisheries and Marine Science Faculty, Universitas Riau

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1904113728

Penerbit

Pekanbaru : Universitas Riau – Fakultas Perikanan Dan Ilmu Kelautan – Teknologi Hasil Perikanan., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1904113728

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNOLOGI HASIL PERIKANAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

Versi lain/terkait

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