CD Skripsi
Valorisasi Cangkang Kerang Kijing (Pilsbryoconcha sp.) Menjadi Hidroksiapatit Sebagai Antibakteri Lactobacillus acidophilus dan Pseudomonas aeruginosa
ABSTRACT
The freshwater of mussel shell (Pilsbryoconcha sp.) are a solid waste that is underutilized in Riau Province. Even though the fresh water of mussel shells are composed of high levels of calcium, the calcium content is 61.39%. So, there is a need for valorization that reduces solid waste. Valorization in this research was used as hydroxyapatite (HAp) bioceramic which contains calcium oxide precursors which have antibacterial properties. This research aims to determine the antibacterial activity of the hydroxyapatite synthesis of mussel shells. The research method used was experimental, namely the synthesis of hydroxyapatite by calcining at a temperature of 1000oC. The test parameters carried out were yield, analysis of the antibacterial activity of Lactobacillus acidophilus and Pseudomonas aeruginosa as well as analysis of MIC and MBC. The synthesis of hydroxyapatite had a yield of 37.65% and was unable to inhibit L.acidophilus and P.aeroginosa bacteria. The diameter of the inhibition zone for the synthesis of hydroxyapatite from mussel shells against the bacteria L.acidophilus and P.aaeroginosa was highest in the 50 mg/mL treatment respectively at 3.66 and 5.04 mm which was classified as very weak. The MIC value of hydroxyapatite against L. acidophilus bacteria and P. aeruginosa bacteria could not be found so the MIC test was not carried out.
Keywords: Gram negative bacteria, Gram negative bacteria Synthesis, Hydroxyapatite, Valorization, Inhibition zone.
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