CD Skripsi
Aktivitas Antibakteri Hidroksiapatit Cangkang Kerang Kijing (Pilsbryochoncha sp.) Terhadap Bakteri Escherichia coli dan Streptococcus mutants
ABSTRACT
Freshwater mussel shell have a high calcium content in their shells, namely 61.39% and have the potential to be a source of hydroxyapatite. Hydroxyapatite is an inorganic mineral with the chemical formula Ca10(PO4)6(OH)2 which can be synthesized from calcium-rich components. Hydroxyapatite is composed of calcium and phosphate which is usually used as bone scaffolding such as bone implants, bone and dental fillers. Calcium and phosphate compounds also play a role in preventing caries. This research aimed to determine the yield, MIC and MBC and determine the zone of inhibition against E. coli and S. mutants bacteria from hydroxyapatite of mussel shells. This research used a non-factorial Completely Randomized Design (CRD) experimental method with 3 treatment levels, namely 5%, 2,5% and 1,25%. The method used to obtain hydroxyapatite is the calcination method. The parameters used in this research were yield, MIC and MBC and inhibition zone. The yields of shell flour, CaO flour and hydroxyapatite were respectively 80.33%, 50.23% and 80.62%. The inhibitory power of hydroxyapatite against E. coli bacteria at concentrations of 5%, 2,5%, 1,25% were 6.59 mm, 2.52 mm, and 0 mm, respectively. However, S. mutants bacteria did not have inhibitory power at concentrations of 5%, 2,5% and 1,25%. The MIC value of hydroxyapatite against E. coli bacteria is present at a concentration of 2.5% with a minimum resistance of 2.52 mm and a MBC value at a concentration of 5% with a minimum suicide of 6.59%. Whereas in S. mutants bacteria there are no values of KHM and KBM.
Keywords: Antibacterial, Phosphate, Hydroxyapatite, Dental caries, Calcium
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