CD Skripsi

Karakterisasi Dan Identifikasi Bakteri Penghasil Dekstran Asal Matoa Dan Kopi

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Dextran is a polymerization of glucose resulting from the decomposition of sucrose by the enzyme dextransucrase, secreted due to bacterial activity. Dextran has potential as a nano-based drug delivery method due to its high solubility, biocompatibility, biodegradability, and non-immunogenicity. This study focuses on the characterization and identification of dextran-producing bacteria isolated from matoa and coffee fruits. Two bacterial isolates were rejuvenated and then phenotypically characterized. Isolate MTM3 has morphological characteristics of circular colonies, an entire, moderate, convex, semi-transparent color, Gram-positive bacillus-shaped shape with a length of 2.21-6.59 μm and a width of 1.40-2.46 μm, does not grow at 4°C and 60°C, and is able to grow at NaCl levels of 2-8% and pH 5. Meanwhile, KPL3 has morphological characteristics of circular colonies that are entire, small, flat, milky white, Gram-positive bacillus-shaped, with a length of 3.06–6.74 μm and a width of 1.0–1.98 μm. Isolate MTM3 reacted positively to the methyl-red test and was able to ferment 12 carbohydrate substrates and KPL3 13 carbohydrate substrates. Based on 16S rRNA sequence analysis, MTM3 isolate is 1421 bp and KPL3 is 1429 bp, and phylogenetic tree construction shows MTM3 and KPL3 isolates are closely related to Bacillus gobiensis strain FJAT-4402 with a p-distance value of 0.042.
Key findings: Bacillus gobiensis, coffee, dextran, dextran-producing bacteria, matoa.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003113102

Penerbit

Pekanbaru : Universitas Riau FISIPOL Biologi., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003113102

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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