CD Tesis

Biotransformasi Zat Warna Bromocresol Purple dengan Kultur Hidup dan Enzim Lakase Semi Murni Trichoderma asperellum LBKURCC1

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The purpose of this work is to ascertain how incubating a live culture and semipure laccase enzyme generated by a local isolate of Trichoderma asperellum LBKURCC1 from Riau, Indonesia, affects the biotransformation of the bromocresol purple (BCP) dye. Using a UV-Vis Spectrophotometer, absorbance of BCP was determined at 0, 3, 6, 9, and 24 hours at pH 5.2; 5.7, and 6.4 while the cultures were being incubated. BCP solution at pH 5.2 incubated with live culture had a color shift from yellow to purple after 24 hours of incubation at λ = 588 nm, with a biotransformation multiple of 7 times. No biotransformation of a BCP was incubated with live cultures at pH 5.7 and 6.4. As observed based on the absorbance spectrum of BCP with live culture at pH 5.2 showed increase the peak at λ = 588 nm. Using a UV-Vis Spectrophotometer, absorbance of BCP was measured every 24 hours at pH 5.5 while it was incubated with semipure laccase. Semipure enzyme produced laccase A and laccase B, however, laccase A was pure than laccase B. On day 8, the hue changed from yellow to purple due to incubation with a laccase A enzyme concentration of 14 U/L, and the BCP biotransformation multiple of 10 times at λ = 588 nm. Conversely, on day 7, biotransformation of BCP with biotransformation multiple was 6 times at λ = 588 nm due to incubation with a laccase B enzyme concentration of 14 U/L. These data showed that the laccase that Trichoderma asperellum LBKURCC1 produces can be utilized as a biotransformation of BCP dye.
Key word : Trichoderma asperellum LBKURCC1, laccase enzyme, Bromocresol Purple, biotransformation

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1910247802

Penerbit

Pekanbaru : Universitas Riau Pascasarjana Tesis Teknik Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1910247802

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

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