CD Skripsi

Produksi, Karakterisasi Pigmen Dari Bakteri Endofit Paku Laut (Acrostichum Aureum L.) Dan Identifikasi Sekuens Gen 16s Rrna

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Endophytic bacteria of sea ferns, namely A.A2 1, A.T 2, A.T2 1, B.A2 1, B.T2 1, D.A2 4, D.A2 6, D.B2 2, and D.B2 3 are known to produce natural pigments. The purpose of this study is the production and characterization of pigments from endophytic bacteria of sea ferns and the identification of 16S rRNA gene sequences. Pigment characterization was done by looking at the maximum wavelength absorption using UV-Vis spectrophotometry, using liquid chromatography-mass spectrometry (LC-MS) analysis, and using 16S rRNA gene sequence identification. The results showed that two of the nine isolates of sea fern endophytic bacteria have the potential to produce pigments with maximum absorption of isolate A.T 2 at 317, 322, and 329 nm and isolate B.A2 1 at 536 nm. The results of LC-MS analysis also showed that isolate A.T 2 has pyocyanin pigment with a molecular weight of 200.11 m/z (C13H13NO (3- (benzyloxy)aniline)) and 179.05 m/z (C12H8N2 (phenazine)) while isolate B.A2 1 is known to have prodigiosin pigment with a molecular weight of 324.21 m/z (C20H25N3O (prodigiosin)). Identification of gene sequences 16S rRNA result for isolate A.T 2 showed 99.93% homology with Pseudomonas aeruginosa strain M4. Isolates A.T 2 and B.A2 1 can be developed as natural dye producers and therapeutic agent innovations.

Key findings: metabolomics, LC-MS, sequencing, UV-Vis

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003113080

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003113080

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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