CD Skripsi
Analisis Gen 16s Rrna Dan Gen Penyandi Transglutaminase Dari Bakteri Laut
The marine environment covers more than 95% of the biosphere and covers more than 70% of the earth's surface. Environmental factors such as temperature, pH and salinity produce promising marine biological resources, causing various microorganisms to live in them, one of which is actinomycetes. Actinomycetes have potential for the industrial world as producers of the enzyme transglutaminase. Transglutaminases are used in the manufacture of surimi, processed fish products, stabilizers of wool and leather products. This study aims to explore actinomycetes as a source of transglutaminase enzyme production by screening, gene detection, and molecular identification of 16S rRNA genes. Screening was carried out using a Paper Disc Filter (FPD) by observing color variations formed, detection of transglutaminase genes, and analysis of the diversity of transglutaminase-producing actinomycetes. The results of the study obtained 40 selected isolates resulting in light brown, dark brown and milk chocolate colors. Isolates SPG22-013, SPG22-031 and DR-015 carried the transglutaminase encoding gene. Isolate SPG22-013 has a close relationship with Mycolicibacterium poriferae with 99% homology, SPG22-031 has 99.8% homology with Rothia kristinae, and DR-015 was identified as Dermacoccus nishinomiyaensis with 99% homology.
Key findings: Actinomycetes, gene transglutaminase, gene 16S rRNA, transglutaminase.
Tidak tersedia versi lain