CD Skripsi

Analisis Sekuens Barkode Dna Rbcl Dan Its Pada Tumbuhan Rotan Pahit (Calamus Caesius Blume)Asal Riau

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Bitter rattan is a clumping and climbing plant. The distribution area of bitter rattan ranges from lowlands to mountains with altitudes of 300-600 meters above sea level, in wet climates. DNA barcoding is a short sequence that can be used in DNA barcoding techniques. This research aims to analyze the DNA barcode sequences of rbcL and ITS from the bitter rattan plant (Calamus caesius Blume) from Riau. The working procedure begins with morphological analysis, followed by molecular analysis, which includes sample collection, total DNA extraction of the bitter rattan plant, total DNA electrophoresis, DNA amplification with PCR, PCR product electrophoresis, sequencing, and data analysis using the BioEdit, BLASTn, and MEGA version 6.0 software. Morphological observations indicate that bitter rattan has stems covered with triangular, flat, brownish-black spines, leaves also covered with fine spines, and oval-shaped fruits. The length of the rbcL DNA sequence in the three bitter rattan plant samples obtained was 840 bp, and for ITS, the sequencing results were poor and thus unreadable. BLASTn analysis of rbcL identified Calamus caryotoides with a 100% identity value with the studied bitter rattan sample. The genetic distance matrix values ranged from 0.000 to 0.006, with the lowest value found in Calamus caryotoides at 0.000 and the highest in Salacca ramosiana at 0.006. The rbcL DNA sequence of bitter rattan from Riau obtained in this study is the first sequence and is not yet in GenBank. Calamus caesius Blume and Calamus caryotoides are not the same species as they have different morphologies.

Key Findings: Barcode DNA, bitter rattan, Calamus caesius Blume, ITS, rbcL.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003126631

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003126631

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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