CD Skripsi

Isolasi Bakteri Asam Laktat (Bal) Kandidat Probiotik Dari Tempe Dan Uji Aktivitas Antibakteri Terhadap Bakteri Patogen

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Lactic acid bacteria (LAB) are bacteria that are safe for human consumption. Tempeh is a substrate that can be metabolized by lactic acid bacteria (LAB) so that it can be utilized to maintain the balance of intestinal microflora. The probiotic properties of LAB contained in tempeh need to be tested biochemically. The purpose of this study was to isolate LAB from tempeh and determine the potential of LAB as probiotic candidates. LAB isolation from tempeh, purified isolates were characterized macroscopically, microscopically and biochemical tests (Gram staining, catalase test, and motility test), probiotic candidate test (salt content resistance test, and pH resistance test, and antibacterial activity test against Staphylococcus aureus ATCC 6538 and Eschericia coli ATCC 35128). The results of LAB isolation obtained eight isolates with macroscopic characters of circular colonies, white and yellowish bacterial colony colors, convex and umbonate elevations, entire edges. Microscopic characterization and biochemical tests of LAB isolates are Gram-positive, bacillus cell shape, catalase negative, and non-motile. The probiotic candidate test of six LAB isolates from tempeh was able to grow at 5% and 6,5% NaCl salt levels, and was resistant to pH 2 and pH 3. Antibacterial activity test of six LAB isolates against S.aureus ATCC 6538 bacteria showed inhibition activity ranging from 7,15 ± 0,17 – 9,57 ± 0,38 mm. Antibacterial activity of six LAB isolates against E.coli ATCC 35128 bacteria showed antibacterial activity with an average inhibition zone ranging from 6,62 ± 0,10 -13,12 ± 0,17 mm. The six LAB isolates have antibacterial ability against pathogenic bacteria so they have potential as probiotic candidates.
Key findings: Lactic acid bacteria, tempeh, probiotic candidate, antibacterial activity, Staphylococcus aureus, Escherichia coli.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003135002

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003135002

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

mardiah

Versi lain/terkait

Lampiran Berkas

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