CD Tesis

Uji mekanisme kerja antibakteri Turunan diterpen kuinon pada bakteri Gram positif dan negative Studi In Vitro Dan In Silico

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Introduction: Diterpene quinone-derived compounds are proven to have antibacterial activity. However, their mechanism of action is unknown. This study aims to determine the mechanism of antibacterial action of a diterpene quinone derivatives (67DOR, 6O7AR) on S. aureus and E. coli through in vitro and in silico assays.
Methods: This study is a laboratory experimental through in vitro and in silico assays. MIC tests were performed using microdilution, and MBC determinations were carried out on MHA media. In vitro assays were conducted using membrane permeabilizing agents including Tris, Triton X-100, and ATPase inhibiting agent with NaN3. In silico assays with molecular docking method was performed on PDB ID 2XCT and 4WF9 proteins in S. aureus bacteria and PDB ID 7P2W and 3J7Z proteins in E. coli bacteria using AutoDock Vina.
Results: MIC and MBC results of compounds 67DOR and 6O7AR on both bacteria were 300 μM and 2400 μM, respectively. In vitro assays result suggested that the antibacterial activities of 67DOR and 6O7AR were associated with the inhibition of ATPase function and disrupting membrane function. Docking results showed that both compounds possessed good interaction with the 2XCT and 4WF9 proteins of S. aureus as well as with the 7P2W and 3J7Z proteins of E. coli.
Conclusion: 67DOR and 6O7AR exhibited good antibacterial activity. Based on in vitro and in silico assays, the mechanism of action of both compounds is associated with disruption of membrane function, inhibition of ATPase, inhibition of gyrase enzymes and 50S ribosomes in S. aureus and E. coli bacteria.
Key words:Diterpene quinones, Gram positive bacteria, Gram negative bacteria, in silico, in vitro, mechanism of action.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2110247768

Penerbit

Pekanbaru : Universitas Riau Pascasarjana Tesis Ilmu Biomedis., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2110247768

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Ilmu Biomedis

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

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