CD Skripsi
Perbanyakan Pisang Kepok (Musa Paradisiaca L.) Dengan Penambahan 6-Benzyl Amino Purine (Bap) Dan Thidiazuron (Tdz) Secara In-Vitro
The propagation of pisang kepok using seedlings derived from its parents is an obstacle for farmers to produce large scale and quality seedlings. In vitro culture is a solution for banana seedling propagation. The growth rate of plants can be supported by the provision of cytokinin type growth regulators (ZPT) such as 6-Benzyl Amino Purin (BAP) and Thidiazuron (TDZ). This study aims to determine the effect of BAP and TDZ on shoot propagation from pisang kepok (Musa paradisiaca L.) explants in vitro and determine the effective concentration of BAP and TDZ for shoot propagation. This study used a completely randomized design (CRD) with 5 (five) treatments levels repeated 5 (five) times resulting in 25 experimental units. Each treatment added 0.1 mg/L TDZ with different concentrations of BAP. The treatments consisted of control (B0), 0.3 mg/L BAP (B1), 0.6 mg/L BAP (B2), 0.9 mg/L BAP (B3) and 1.2 mg/L BAP (B4). The parameters observed were the percentage of living explants, time appeared shoots, time appeared nodules, percentage of shoots formed, percentage of nodules formed, number of shoots and number of nodules. The results of this study show that the concentration of 0.9 mg/L BAP (B3) is the best treatment forms the highest namely 9 shoots. The highest number of nodules resulted from treatment of 0.6 mg/L BAP (B2) with 7 nodules. Different concentrations of BAP added with TDZ influenced the formation of engagement shoots and nodules.
Key findings: BAP, TDZ, in vitro, shoots propagation, Musa paradisiaca L.
Tidak tersedia versi lain