CD Skripsi
Struktur Anatomi Dan Uji Histokimia Daun Merampuyan (Rhodamnia Cinerea Jack)
Merampuyan (Rhodamnia cinerea Jack) is a species of the Myrtaceae family that has a tree habit and with an average height of 5-6 meters and has simple leaves. Merampuyan leaves have medicinal properties. This research aims to characterize the anatomical structure of merampuyan leaves and identify and determine the location of secondary metabolite compounds contained in them. The samples taken were leaves bearing the fifth node. This research was conducted from December 2023 to May 2024. Anatomical preparations are made using the paraffin method, observation of epidermal characteristics including stomata and trichomes was carried out using the paradermal method, and histochemical tests are carried out by making fresh incisions and there are four tests including tests for alkaloids, flavonoids, tannins and phenols. The results of anatomical observations show the characteristics of each tissue. The petiole and midrib have bicollateral type transport bundles. The intercostals and leaf margins are composed of mesophyll tissue which is differentiated into palisade and spongy tissue. There are secretion cavities in all four parts. The characteristic of the epidermis of Merampuyan leaves is that they have a polygonal shape. Hypostomatic stomata with parasitic type. The histochemical test results of Merampuyan leaves showed positive results for alkaloids, flavonoids, tannins and phenols. Phenol is found in many midribs, intercostal, margin and petiole. Tannins are found in petiole and midrib, while alkaloids and flavonoids are found in petiole. This study provides additional information about the anatomical structure, histochemistry of merampuyan leaves, as well as revealing the characteristics of the epidermis and stomata of this plant.
Key words: Anatomical structure, histochemistry, Rhodamnia cinerea, stomata.
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