Image of Efek Prakondisi Vitamin D terhadap Karakteristik Biologis dan Ekspresi Tumor Necrosis Factor- Α, Interleukin-6, Vascular Endothelial Growth Factor pada Sel Punca Mesenkimal
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Efek Prakondisi Vitamin D terhadap Karakteristik Biologis dan Ekspresi Tumor Necrosis Factor- Α, Interleukin-6, Vascular Endothelial Growth Factor pada Sel Punca Mesenkimal

VITRIYANNA MUTIARA YUHENDRI. NIM. 2110247804. The Effect of Vitamin D Preconditioning on Biological Characteristics and Expression of Tumor Necrosis Factor-α, Interleukin-6, Vascular Endothelial Growth Factor in Mesenchymal Stem Cells. supervised by Arfianti and Ismawati.
Background: Mesenchymal stem cells (MSCs) have great potential in regenerative medicine applications. Vitamin D has immunomodulatory roles through the interaction with vitamin D receptor (VDR) which is expressed by MSC. The purpose of this study was to determine the effect of vitamin D preconditioning on biological characteristics and expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and vascular endothelial growth factor (VEGF) of MSCs.
Methods: Mesenchymal stem cells isolated from umbilical cord tissue and preconditioned with vitamin D3 concentrations of 0-100 nM for 24 and 48 hours were analyzed for viability by CCK-8 assay, while SPM migration analysis with vitamin D3 preconditioning of 50 nM for 24 and 48 hours was assessed by Scratch wound healing assay. In addition, mRNA expression of TNF-α, IL-6, and VEGF after vitamin D3 preconditioning 50 nM for 48 hours was also measured by quantitative real-time Polymerase Chain Reaction method.
Results: The administration of 50 nM vitamin D3 for 48 hours significantly increased the viability and migration of MSCs. Supplementation of vitamin D3 in MSCs caused a significant (p

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Perpustakaan Universitas Riau 2110247804
2110247804
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Informasi Detail
Judul Seri
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No. Panggil
2110247804
Penerbit
Pekanbaru : Universitas Riau Pascasarjana Tesis Ilmu Biomedis.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
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Klasifikasi
2110247804
Tipe Isi
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Tipe Media
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Tipe Pembawa
-
Edisi
-
Subjek
Ilmu Biomedis
Info Detail Spesifik
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Pernyataan Tanggungjawab
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