CD Skripsi
ISOLASI DAN KARAKTERISASI BAKTERI TERMOFILIK SEBAGAI PENGHASIL ENZIM AMILASE DARI SUMBER AIR PANAS KEPANASAN DI KECAMATAN TAPUNG HULU KABUPATEN KAMPAR, RIAU
Thermophilic bacteria are a group of bacteria that can live and survive at high temperatures ranging from 45°C-80°C. The use of thermophilic bacteria is the best choice as a producer of thermostable enzymes that can survive high temperatures which are used in various industrial fields including the textile industry, food , detergent and pharmaceutical. This study aims to isolate, characterize and test the activity of thermostable amylase enzyme from thermophilic bacterial isolates in Kepanasan hot spring in Tapung Hulu District, Kampar Regency, Riau. Characterization of thermophilic bacterial isolates is macroscopic, physiological and biochemical tests (triple sugar iron agar, sulfur indole motility, symmons citrate, and catalase). The results of the study obtained ten isolates of thermophilic bacteria consisting of six isolates from hot water samples and four isolates from soil sediments isolated at temperatures of 45°C and 50°C using a nutrient agar (NA) medium. The results of Gram staining were obtained one Gram-positive isolate and nine Gram-negative isolates. Positive catalase test results, negative indole and motility tests for ten isolates. The results of the amylase enzyme activity test obtained three bacterial isolates, namely BTA 6, BTT 2 and BTT 4 with the highest amylolytic index of 1.32 produced by BTT 2 isolate at an incubation temperature of 50°C with a starch concentration of 1%.
Key findings: Hot water Kepanasan, thermophilic bacteria, amylase enzymes, amylolytic index
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