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Analisis Metabolomik Untuk Mengevaluasi Pengaruh Pengadukan Fermentasi Dan Jenis Pelarut Ekstraksi Pada Ko-Kultur Penicillium Sp. Lbkurcc34 Dan Staphylococcus Aureus

The potential for discovering new antibiotic alternatives through the exploration of local microbes from Riau as a source of bioactive compounds is significant. However, isolating active compounds using a guided assay approach presents challenges due to the limited amount of extract and the low yield obtained from microbial fermentation. To address these challenges, it is crucial to optimize fermentation production factors, such as the impact of agitation and extraction solvents, to enhance the production of bioactive compounds. This study seeks to assess the effects of agitation fermentation and type of extraction solvents in the liquid fermentation of a co-culture of Penicillium sp. LBKURCC34 and Staphylococcus aureus in the production of antimicrobial compounds and to conduct an analysis of the metabolite profile using an untargeted metabolomics approach based on LC-HRMS Q Orbitrap. The fermentation data underwent analysis using Xcalibur, MS-DIAL, and GNPS software multivariate analysis was employed to identify the bioactive compounds generated. The compounds that were identified included cyclo(phe-pro), cyclo(val-pro), and maculosin, astaxanthin, harmaline, phenalinolactone a, gossypetin, sanguinarine, and dinactin. The results indicated that intensive agitation treatments yielded a different compound composition compared to non-agitation treatments. Furthermore, technical-grade and pro-analysis solvents did not result in differences in the extracted compound composition in non-agitated treatments but showed variations in agitated treatments. Several identified bioactive compounds exhibit potential for further development.  

Ketersediaan
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Perpustakaan Universitas Riau 2003112906
2003112906
Tersedia
Informasi Detail
Judul Seri
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No. Panggil
2003112906
Penerbit
Pekanbaru : Universitas Riau FMIPA Kimia.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
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Klasifikasi
2003112906
Tipe Isi
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Tipe Media
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Tipe Pembawa
-
Edisi
-
Subjek
KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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Tidak tersedia versi lain

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