CD Skripsi
Uji Potensi Metabolit Sekunder Dari Ekstrak Umbi Bawang Merah (Allium Cepa Linn) Sebagai Antioksidan Dan Inhibitor Enzim Α-Amilase
Some studies inform that Allium cepa L. onions contain secondary metabolites that have the potential as natural antioxidants and as inhibitors to inhibit α-amylase activity. Therefore, the antioxidant potential and α-amylase inhibition of three different types of shallots, namely onions, Brebes and Solok were tested and it is suspected that these three shallots contain different secondary metabolites. In this study, three shallot bulbs in fresh and dried conditions were extracted with n-hexane and water solvents, respectively. The variables tested were taxonomy, water content of fresh onion, and for the extract, the percentage yield, phytochemical test, IC50 antioxidant using DPPH method and IC50 α-amylase using DNS method were determined. The results of determining the moisture content of fresh onions from onions, Brebes red and Solok red were 86.96 ± 0.01674%, 83.44 ± 0.00058% and 84.28 ± 0.02610% respectively. The percentage yield of n-hexane extract ranged from 19.9 - 54.7% and aqueous extract 54.1 - 71.6%. Phytochemical tests showed positive alkaloid, phenolic and terpenoid compounds in all extracts, while saponins were only positive in aqueous extracts and flavonoids were positive in aqueous extracts and n-hexane extracts of fresh Brebes and Solok onions. Extracts that have potential as antioxidants are only water extracts from dried onions with an IC50 value of 129.02 ppm in the medium category with secondary metabolite content, namely alkaloids, phenolics, saponins and steroids, while other extracts have IC50 > 200 ppm. The IC50 value of α-amylase enzyme inhibition of the three test extracts (water extracts of fresh bombay, dried Brebes and dried Solok) was not potential as an α-amylase inhibitor because the IC50 value was > 100 ppm
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