CD Skripsi
Aktivitas Enzim Selulase Dari Beberapa Isolat Bakteri Selulolitik Dan Kemampuannya Dalam Mendekomposisi Limbah Organik
Agricultural and plantation waste is organic waste that is organic in nature. Generally, this waste is difficult to manage. One alternative for managing agricultural and plantation waste is through biological composting using cellulolytic bacterial bioactivators. Cellulolytic bacteria are bacteria that can produce cellulase enzymes so that they are able to degrade cellulose through the process of breaking down cellulose into a simpler compound, namely glucose. This research aims to determine the cellulase enzyme activity of several isolates of cellulolytic bacteria and their ability to decompose organic waste. The study used a two-factor factorial completely randomized design. The first factor is the type of bacteria, namely Bacillus cereus JP6, Bacillus cereus JP7, Proteus mirabilis Oil palm empty fruit bunches 3, Proteus mirabilis Oil palm empty fruit bunches 7, Providencia vermicola SA1, Bacillus cereus SA6 and consortium. The second factor is the type of organic waste, Oil palm empty fruit bunches, solids and rice straw. The two factors were combined, 21 combinations were obtained and repeated 2 times so that there were 42 experimental units. The parameters observed were cellulase enzyme activity test analysis, weight loss of compost material, water content, odor, color and texture of compost, pH measurements, organic C content, total N analysis, total P analysis, total K analysis and C/N ratio, The results were analyzed using variance and further DNMRT tests at the 5% level. The results of the research showed that the highest cellulase enzyme activity was JP6 bacteria and the administration of single cellulolytic bacteria was able to produce compost results that were relatively similar to the compost results provided by a combination of bacteria isolates, namely consortium.
Keyword: Bacillus cereus, Proteus mirabilis, water content, rice straw
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