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Efek Kombinasi Hipoksia dan Interferon-ℽ terhadap Viabilitas, Migrasi dan Ekspresi Gen Faktor Pertumbuhan pada Sel Punca Mesenkimal

Background: Mesenchymal stem cells (MSCs) have great potential for therapy due to their self-renewal, differentiation and immunoregulatory capacities. The proliferative and functional activity of MSCs as well as its immunomodulatory properties can be impaired as a result of oxidative stress-induced senescence during long-term culture of MSCs. Modification of MSCs culture is very important to improve the therapeutic efficacy of MSCs. The aim of this study was to determine the effects of hypoxia and IFN-ℽ in combination on viability, migration and expression of transforming growth factor-β (TGF-β), hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) genes of MSCs.
Methods: Mesenchymal stem cells were isolated from Wharton’s jelly umbilical cord tissue and cultured under normoxia conditions (control), hypoxia conditions (100 M CoCl2) or supplementation of 50 ng/mL IFN-ℽ, or combination of hypoxia and IFN-ℽ for 24 and 48 hours. The cell viability was examined by CCK-8 assay and migration rate of analysed using scratch wound healing assay with or without serum in the culture medium. In addition, the expression of TGF-β, HGF and VEGF mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR) method.
Results: While the combination of hypoxia and IFN-ℽ for 24 hours did not affect the viability of MSCs, this treatment for 48 hours decreased cell viability compared to control group (p

Ketersediaan
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Perpustakaan Universitas Riau 2110247771
2110247771
Tersedia
Informasi Detail
Judul Seri
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No. Panggil
2110247771
Penerbit
Pekanbaru : Universitas Riau Pascasarjana Tesis Ilmu Biomedis.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
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Klasifikasi
2110247771
Tipe Isi
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Tipe Media
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Tipe Pembawa
-
Edisi
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Subjek
Ilmu Biomedis
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Pernyataan Tanggungjawab
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