CD Skripsi
Isolasi Dan Identifikasi Bakteri Pendegradasi Mikroplastik Pada Sedimen Di Perairan Bagansiapiapi Provinsi Riau
Microplastic pollution in the marine environment has become a critical environmental issue that requires a biological remediation approach. The coastal waters of Bagansiapiapi, Riau Province, are an important fishery area potentially affected by microplastic contamination. This study aims to isolate and identify microplastic-degrading bacteria from sediments in the waters of Bagansiapiapi, Riau Province. Sediment samples were collected from three stations representing different environmental conditions. Bacterial isolation was carried out using selective media, followed by biochemical identification tests including Gram staining, catalase, oxidase, motility, indole, methyl red, and H2S tests. Water quality parameters and microplastic abundance were also measured. Eleven bacterial isolates (ISD 1-11) were successfully isolated from sediment samples. Morphological analysis showed that the diameter of the bacteria ranged from 0.1-1.2 cm, with predominantly white color and round shape. Biochemical tests showed that 81.8% of the isolates were Gram-positive cocci, while 18.2% were Gram-positive bacilli. All isolates showed positive results for catalase and oxidase, with variable results for motility, indole, methyl red, and H2S tests. Bacterial abundance varied significantly between stations, with Station I showing the highest mean (24.9×104 CFU/ml), followed by Station III (33.1×104 CFU/ml) and Station II (17.9×104 CFU/ml). The abundance of microplastics ranged from 0.02-0.08 particles/m³ at all stations.
Keywords: bacteria, biodegradation, microplastic, sediment.
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