CD Skripsi
Pengaruh Penggunaan Suhu Deasetilasi Berbeda Terhadap Mutu Kitosan Cangkang Kepiting Bakau (Scylla Serrata)
This research aims to obtain the best temperature in the chitosan extraction process, to know the quality of chitin and chitosan products. This research was conducted in three stages, that is: 1) Preparation and proximate analysis of mud crab shell flour, 2) chitin extraction and 3) chitosan extraction. The experimental design used for chitosan isolation was Completely Randomized Design (RAL). The process of deacetylation chitin becomes chitosan by using 50% NaOH with varying heating temperature (120, 130, and 140°C). Parameters observed were yield, moisture content, ash content and degrees of deacetylation. The result showed that the chemical compositions contained in the mud crab shell flour were: moisture content 5.38% (gw), ash 57.26% (dw), fat 2.38% (dw), protein 13.62% (dw) and carbohydrate 28,67% (by difference). The best chitosan was obtained by chitin deacetylation process into chitosan using temperature of 130°C (KO2). Characteristics quality of chitosan mud crab shell produced are KO1: yield 61.00%, moisture content 6.47%, ash content 17.18% and degree of deacetylation 49.63%. KO2: yield 59.94%, moisture content 6.48%, ash content 14.85% with degree of deacetylation 51.13%. KO3: yield 53,97, moisture content 6.54%; ash content and degree of deacetylation14.66%, 52.63%. Characteristic quality of chitin included yield was 27.81%, moisture content 7.29%, ash 44.05% and degree of deacetylation 33.09%.
Keyword: Chitosan, Chitin, Degree of deacetylation, Mud crab shell
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